Detection of Gene p/tms121  for Photoperiod and ThermoSensitive Genic Male Sterility by TetraPrimer Amplication Refractory Mutation System PCR in Rice

doi:10.3969/j.issn.1001-7216.2014.04.014

Abstract

Abstract: Specific primers were designed for tetraprimer amplification refractory mutation system PCR (ARMSPCR) method according to the single nucleodide mutation in gene p/tms121. Eleven twoline sterile lines and two normal varieties were analyzed by this method. The results could clearly distinguish the genotype of p/tms121, which was completely consistent with the result of CAPs markerbased analysis. Therefore, as a lowcost, simple and reliable technique, this method could be widely used to the p/tms121 detection and molecular markerassisted breeding in rice.

Key words: rice, PTGMS, p/tms121, tetraprimer ARMSPCR

摘要： 根据p/tms121基因存在的单核苷酸变异，利用四引物扩增受阻突变体系PCR(Tetraprimer ARMSPCR)的方法设计特异引物，对11个两系不育系水稻品种（或品系）以及2个常规稻品系进行扩增。根据其PCR产物带型，可以准确鉴定光温敏核不育基因p/tms121的基因型，其检测结果与通过CAPS标记检测的结果完全一致。该方法成本低、简单、可靠，可用于p/tms121基因的鉴定和分子标记辅助育种。

关键词: 水稻, 光温敏核不育, p/tms121基因, 四引物扩增受阻突变体系PCR

CLC Number:

Q343.5
A755

LI Jun*, LI Bai, GAO Rongcun. Detection of Gene p/tms121 for Photoperiod and ThermoSensitive Genic Male Sterility by TetraPrimer Amplication Refractory Mutation System PCR in Rice[J]. Chinese Journal of Rice Science, 2014, 28(4): 442-446.

李军* ,李白,高荣村. 利用四引物扩增受阻突变体系PCR技术检测水稻光温敏核不育基因p/tms12-1[J]. 中国水稻科学, 2014, 28(4): 442-446.

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Detection of Gene p/tms121 for Photoperiod and ThermoSensitive Genic Male Sterility by TetraPrimer Amplication Refractory Mutation System PCR in Rice

利用四引物扩增受阻突变体系PCR技术检测水稻光温敏核不育基因p/tms12-1

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